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The principal achievement with both steroids was again the C1-2 double bond, which markedly increases the ratio of anabolic to androgenic effect in each case. The increase in the C1-2 double bond in both testosterone and dihydrotestosterone is in the direction of the C1-2 double bonds in the dihydrotestosterone. This was previously demonstrated in some studies with steroidal and growth hormone receptors (5, 6, 17). Although the C1-2 double bond is a general enhancement in the affinity of the receptor for the steroidal or growth hormone, when both steroids are present, the C1-2 double bond also increases the affinity of the receptor for the testosterone and a secondary increase in the affinity for the Growth hormone (5). The ratio of C1-2 dual bonds in testosterone or dihydrotestosterone to that in dihydrotestosterone is typically very variable. In dihydrotestosterone the ratio is normally 1:1, so that the binding with testosterone to the receptor is increased to a value greater than 1 (18). In the case of testosterone the ratio is typically 1:2; in dihydrotestosterone the ratio is usually 1:6, so that the binding is decreased to a value lower than 1 (5, 6, 7, 18). The ratio of anabolic to androgenic effect with testosterone and dihydrotestosterone are normally greater than 1. In our study we obtained for each steroid the highest and lowest values (Fig. S3). It should be noted that there is also a large variation in the anabolic to androgenic ratios in this same group over the studies (Table S2). One of the possible explanations of the differences in this group is that in contrast with studies in men, in both the normal young adult and elderly men there is no effect of chronic supplementation with either testosterone or dihydrotestosterone on the ratio of anabolic to androgenic hormone. However, in the elderly we observed that a single dose of testosterone (1, 500 mg) did not produce a significant increase in the ratio in subjects aged over 60. Furthermore, with dihydrotestosterone there was no change observed in the ratio in subjects aged over 90 that has been observed in subjects who are not receiving chronic or seasonal oral steroids such as the diuretics and statins. These data indicate that steroidal and metabolic stimulation of both growth and anabolism in both men and adolescents is not limited to growth hormone and dihydrotestosterone.The effect in our study of the C1-2 triple bond on the binding of the DHT and its receptor (17, 19)Similar articles: